Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193515_2wyp ACCTTGTGAACTGAACTGCAGCTCAGAGCATG(+CAGTGT)GATCCCTTAAACTAAAGCGATGGTCTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605193513_vkla CCTGCATGACAGACCATCGCTTTAGTTTAAGG(+CAGTGT)GATCCATGCTCTGAGCTGCAGTTCAGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605193512_yvkq TATATTATAATGCTATTGTTGCGAGCGCTATG(+CAGTGT)GATCCCCTTTTCACGCCATCCTGCTGACCGG NGG 10 8 17 8 24 0 100 5 10
    240605193510_8ixj TATTGCCGGTCAGCAGGATGGCGTGAAAAGGG(+CAGTGT)GATCCATAGCGCTCGCAACAATAGCATTATA NGG 10 8 17 8 24 0 100 5 10
    240605193508_9pfi ACTGGCTAAGCACACATACTGCTCTTTCAGAG(+CAGTGT)GATCCAAGTTCAGTTCCAAGAACCCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605193507_wsll CTGCTCAAAGGGGGTTCTTGGAACTGAACTTG(+CAGTGT)GATCCTCTGAAAGAGCAGTATGTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605193505_empy GCCCTGGTTGAGTGTCGTGTGTCACACCTGTG(+CAGTGT)GTACCTGCTCAGATTTATTTCACTTCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605193504_vi65 GTAAACCAAAGAAGTGAAATAAATCTGAGCAG(+CAGTGT)GTACCACAGGTGTGACACACGACACTCAACC NGG 10 8 17 8 24 0 100 5 10
    240605193503_76ou AGCCGAACCACAGATGGCTTCGAGTAAGTCTG(+CAGTGT)GATCCTGACACCCGCCCTCCCCACCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240605193501_erhi CTGAAGCTCGGGGTGGGGAGGGCGGGTGTCAG(+CAGTGT)GATCCAGACTTACTCGAAGCCATCTGTGGTT NGG 10 8 17 8 24 0 100 5 10