Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193445_iih1 GATCCACATGGAAGGCTCCAACAGCAACAAGG(+CAGTGT)GATCCATGCAGCCTGCAGAGAGCTTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605193444_c4vg GAAAAGTAGAAAAGCTCTCTGCAGGCTGCATG(+CAGTGT)GATCCCTTGTTGCTGTTGGAGCCTTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605193442_r9jm TGGGATGTTTTTTAGGTCTTTGCTATTGCAGG(+CAGTGT)GTACCATCCCTTTGAAGCCCTTTCCTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605193440_oznd CAAAAACAGGAGGAAAGGGCTTCAAAGGGATG(+CAGTGT)GTACCCTGCAATAGCAAAGACCTAAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240605193439_vun2 TCAAGGTCAAAGAAACATAGAAGGGTAAGAGG(+CAGTGT)GTACCCTGTGAAATGATCCTTTCATGCACCT NGG 10 8 17 8 24 0 100 5 10
    240605193437_0okg AGAGAAGGTGCATGAAAGGATCATTTCACAGG(+CAGTGT)GTACCCTCTTACCCTTCTATGTTTCTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605193436_ie9u AGTGACAGATTAACAGGGGTAGCTGCTACTTG(+CAGTGT)GATCCACAGAATCTCTGCAAGTGAGGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605193434_39ee CATAGTTAACCCTCACTTGCAGAGATTCTGTG(+CAGTGT)GATCCAAGTAGCAGCTACCCCTGTTAATCTG NGG 10 8 17 8 24 0 100 5 10
    240605193433_xcvf CAGCCCCAGCAAGCGCCCAGGAACAGGGAGAG(+CAGTGT)GATCCATGCATGTGAGCAGCCAGCATCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605193432_je15 GGTGTCCTTGATGCTGGCTGCTCACATGCATG(+CAGTGT)GATCCTCTCCCTGTTCCTGGGCGCTTGCTGG NGG 10 8 17 8 24 0 100 5 10