Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194550_70pp CTGGTGACAAATGGCAGATGTGCCATATATGG(+CAGTGT)GATCCTTTACTTGGAAAGAAATTAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605194549_cwn1 CCTTCCCCTGACTAATTTCTTTCCAAGTAAAG(+CAGTGT)GATCCCATATATGGCACATCTGCCATTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605194547_hir5 TGGGATGTGGAGAGAAGGTATTCTATGAGTGG(+CAGTGT)GTACCACTGTTAGGACATGGGGGTCGGGGCG NGG 10 8 17 8 24 0 100 5 10
    240605194545_kjhv TTTACCGCCCCGACCCCCATGTCCTAACAGTG(+CAGTGT)GTACCCACTCATAGAATACCTTCTCTCCACA NGG 10 8 17 8 24 0 100 5 10
    240605194544_q8x3 GGGATTATATGATGGGCTGGAGGAGTTTGGAG(+CAGTGT)GATCCCCATTCAGAGTGGGGCCTAGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194542_e7co ACAACCAGGCCCTAGGCCCCACTCTGAATGGG(+CAGTGT)GATCCTCCAAACTCCTCCAGCCCATCATATA NGG 10 8 17 8 24 0 100 5 10
    240605194540_wugk TAGTAATAGAACAGTGATTAAGACAAAAGTTG(+CAGTGT)GTACCAAGGAGCAAAGTATTCTATGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605194539_6jor GTTAGGCTTTTCATAGAATACTTTGCTCCTTG(+CAGTGT)GTACCAACTTTTGTCTTAATCACTGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605194537_uq4r ATGGATTTCTTACTCCTCTTCTCCATGGCTGG(+CAGTGT)GATCCTAGCTTCAGTCCTCATATCTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605194536_e5d2 GGTGAGTAAACAGATATGAGGACTGAAGCTAG(+CAGTGT)GATCCCAGCCATGGAGAAGAGGAGTAAGAAA NGG 10 8 17 8 24 0 100 5 10