Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193254_2w0k ATAAAGTGTTTCTTACTGTTTGTAACCCCCTG(+CAGTGT)GTACCAGTGTGTCCATCTCCAGGCTCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605193253_0fd0 AGAGGGCAAGGAGCCTGGAGATGGACACACTG(+CAGTGT)GTACCAGGGGGTTACAAACAGTAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605193251_nttr GTAAGAAGTCTCCATGGCTCTGGGTCAGCAGG(+CAGTGT)GTACCAGAGATACTGCCCTTGCCAAATACTT NGG 10 8 17 8 24 0 100 5 10
    240605193249_n0wu CCATCAAGTATTTGGCAAGGGCAGTATCTCTG(+CAGTGT)GTACCCTGCTGACCCAGAGCCATGGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605193246_92z1 ATAAAGTGTTTCTTACTGTTTGTAACCCCCTG(+CAGTGT)GTACCAGTGTGTCCATCTCCAGGCTCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605193244_qpsh AGAGGGCAAGGAGCCTGGAGATGGACACACTG(+CAGTGT)GTACCAGGGGGTTACAAACAGTAAGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605193243_5qqq GTGGAAACCTCAGGTTATTGTACTTGTATGAG(+CAGTGT)GTACCCTAGGCAATTCGGCCACTTCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605193240_d580 TCCTGATTTCAGAAGTGGCCGAATTGCCTAGG(+CAGTGT)GTACCTCATACAAGTACAATAACCTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605193238_jbku CCAGTGCCCATAGAAGTCAGAAGAGGGCATTG(+CAGTGT)GATCCTCTGGATCAATGGATTTGGAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605193237_bd3a TCATCCCCAGCTCCAAATCCATTGATCCAGAG(+CAGTGT)GATCCAATGCCCTCTTCTGACTTCTATGGGC NGG 10 8 17 8 24 0 100 5 10