Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193355_fbjm GTCGGGCCCCCAACAACTTGAAGCTTCCTCTG(+CAGTGT)GATCCTCACTTGAGCTCTTTGGGGATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605193353_8cep CCTAGGCCCCATCCCCAAAGAGCTCAAGTGAG(+CAGTGT)GATCCAGAGGAAGCTTCAAGTTGTTGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605193352_t3wj CTATGCTACCCCACTTCCTGTATGCTCAACTG(+CAGTGT)GATCCTCAAGACCATTTCATCTCTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605193350_acst CGTGGTGCAGCTAGAGATGAAATGGTCTTGAG(+CAGTGT)GATCCAGTTGAGCATACAGGAAGTGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605193348_h1wn TGACAGACTTGATGCTTTGAAATTGACCATTG(+CAGTGT)GTACCTCCTCCTTAAAAGAGAAGTGTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240605193347_eren CCTGAACACGACACTTCTCTTTTAAGGAGGAG(+CAGTGT)GTACCAATGGTCAATTTCAAAGCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605193345_yrr4 CTGGGTGTGCAGGAATTCTGCCTGTGAAGTGG(+CAGTGT)GATCCACAGCATGTTCGACGGGTGGGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605193343_p94u GCCCAGTTCTCCCACCCGTCGAACATGCTGTG(+CAGTGT)GATCCCACTTCACAGGCAGAATTCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605193342_f3r4 CTTGTTCTACCATCTCTCCTGGGTTCTTCATG(+CAGTGT)GTACCACCTCGTGTACAGCCACGCCAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605193340_gwnr CTGGTTTAACTGGCGTGGCTGTACACGAGGTG(+CAGTGT)GTACCATGAAGAACCCAGGAGAGATGGTAGA NGG 10 8 17 8 24 0 100 5 10