Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194608_udnl CATACCTGAAATGTAAGTGAAGTCAGCCCATG(+CAGTGT)GATCCCAGAGCAGCCTCACTGTGCATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605194606_onkp AGATCCCACCATGCACAGTGAGGCTGCTCTGG(+CAGTGT)GATCCATGGGCTGACTTCACTTACATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605194605_znul ACATGAGCTCACCAGGAGCTGCTGTTTTCTGG(+CAGTGT)GTACCTTCTGTAGAGAACTTACCCATCACAT NGG 10 8 17 8 24 0 100 5 10
    240605194603_8d2h CACCCATGTGATGGGTAAGTTCTCTACAGAAG(+CAGTGT)GTACCCAGAAAACAGCAGCTCCTGGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605194601_js3b CCTCTAAAGGAGGATTAAGAAGGTAAGAGTTG(+CAGTGT)GTACCTAACCTAGATATGGAAGACTTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605194600_wht5 TATTGTGACTAAGTCTTCCATATCTAGGTTAG(+CAGTGT)GTACCAACTCTTACCTTCTTAATCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605194558_zoxg TTCATCTCTCTGAACACGTTTATCCCAACCTG(+CAGTGT)GATCCAGGCTGCAGTAAGTGCTCAGTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605194557_7jrw GCTCAACCCCACTGAGCACTTACTGCAGCCTG(+CAGTGT)GATCCAGGTTGGGATAAACGTGTTCAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605194554_bqcn ACAGGTAAAGGAATTAGGCTTGGGGGACTTGG(+CAGTGT)GATCCTAGGGAAGAAAGCTCGGGCGATCGGC NGG 10 8 17 8 24 0 100 5 10
    240605194552_4mca ACTCAGCCGATCGCCCGAGCTTTCTTCCCTAG(+CAGTGT)GATCCCAAGTCCCCCAAGCCTAATTCCTTTA NGG 10 8 17 8 24 0 100 5 10