Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192719_8pek AGTCATTTTGAATTTGGTCTGTGCCTTCTCTG(+CAGTGT)GATCCCTCCCTAGCCTGTGCCCCGAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240605192717_4o6i CTATGGACGGCTCGGGGCACAGGCTAGGGAGG(+CAGTGT)GATCCAGAGAAGGCACAGACCAAATTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605192714_4x8n CACCCACTTGAGGTCTCCCGACATTCACCCTG(+CAGTGT)GTACCACACCACTTGATCTCCAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240605192713_ii84 GGGCCAGATTGCTCTGGAGATCAAGTGGTGTG(+CAGTGT)GTACCAGGGTGAATGTCGGGAGACCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605192711_drla TCGCCAGTTCTGTTTGGAGTCCGTGTAGGTGG(+CAGTGT)GATCCTGCTGTGTGTAGCACTGGCCTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605192709_0fzk GGAGATGTCCAGGCCAGTGCTACACACAGCAG(+CAGTGT)GATCCCACCTACACGGACTCCAAACAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605192707_7ch1 TGTAGGGGGTTGAGAGGAAGGGAATGAGCCAG(+CAGTGT)GTACCCTGCATTCCAAGGGAGTTTCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605192705_bqmb CTTTGCACACTGAAACTCCCTTGGAATGCAGG(+CAGTGT)GTACCTGGCTCATTCCCTTCCTCTCAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605192703_afuw GAAGGTTTAGAGAATCCGATGGGCGCTGCCTG(+CAGTGT)GATCCCGGCATAGCCCCAGACACTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605192701_s42l ATAAAGGAACCAAGTGTCTGGGGCTATGCCGG(+CAGTGT)GATCCAGGCAGCGCCCATCGGATTCTCTAAA NGG 10 8 17 8 24 0 100 5 10