Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191448_nfmc CAAGAGTAGAGTTGACCAGAAGGCCTTGGTAG(+CAGTGT)GATCCCTCTAATAATATGTTTGTTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605191446_lqm0 TACATATGCAGCTAGAGACACAAACTCAGGGG(+CAGTGT)GTACCTGTTAGTTCATATTGTTGTTCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605191445_yfeg CCTATAGGAGGAACAACAATATGAACTAACAG(+CAGTGT)GTACCCCCTGAGTTTGTGTCTCTAGCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605191443_enzx TGTGTCTGTCTTTGGTGGTTGATTATGGTATG(+CAGTGT)GATCCCTGGATGGGACAGTCTCTGCATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605191441_cbri GGAAAGACCATGCAGAGACTGTCCCATCCAGG(+CAGTGT)GATCCATACCATAATCAACCACCAAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605191439_vpmz TTTTGGCCCTGTTGATCTCGACTGAATGCCAG(+CAGTGT)GTACCAGTCTTCTCTACCTTCCCTGCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605191437_8uqq TGAGCCTTTGGCAGGGAAGGTAGAGAAGACTG(+CAGTGT)GTACCTGGCATTCAGTCGAGATCAACAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605191435_lzp2 AAAAAGCTGCTTCTAGTGTGTGAGGTTTCCGG(+CAGTGT)GTACCTTTTAGTTCCTCATAAAGTTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605191434_1srm TCAGAGTCTGCAACTTTATGAGGAACTAAAAG(+CAGTGT)GTACCCGGAAACCTCACACACTAGAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605191433_o1pn AAAAGGTTTGTTTTCTTTCATAATTGCATCTG(+CAGTGT)GTACCCAGCAGGAAGTACCTGGCCCCTCCTT NGG 10 8 17 8 24 0 100 5 10