Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191411_4x01 TTACTTCTCTTTTATTTAAAAAGAAATGAGGG(+CAGTGT)GATCCACCTTGGGACAGCACAGCACAACAGT NGG 10 8 17 8 24 0 100 5 10
    240605191409_wmcw GGCTATTCTCATGTGCTGTCACAGGCAGCCTG(+CAGTGT)GATCCTTGGTGGTCAGGGGATGACCCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605191408_jufj AGAGGAGCTTGGGTCATCCCCTGACCACCAAG(+CAGTGT)GATCCAGGCTGCCTGTGACAGCACATGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605191406_nfg4 GGGTCTTACATGCATGTAGGTTTTCCCTAACG(+CAGTGT)GATCCCCGGTGATTAGACCCTTGGACAACAG NGG 10 8 17 8 24 0 100 5 10
    240605191402_686r TTCAGCTGTTGTCCAAGGGTCTAATCACCGGG(+CAGTGT)GATCCGTTAGGGAAAACCTACATGCATGTAA NGG 10 8 17 8 24 0 100 5 10
    240605191400_zyul CCACAATCACACACACTGCCCAGGGACCTCTG(+CAGTGT)GTACCCTAGTGGGTGTGTCTGCCCCATGTCA NGG 10 8 17 8 24 0 100 5 10
    240605191359_dt2z CTTAATGACATGGGGCAGACACACCCACTAGG(+CAGTGT)GTACCAGAGGTCCCTGGGCAGTGTGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605191357_l1bc TCCACCACTTGGAAAGCGGCGGTGACCCAGAG(+CAGTGT)GATCCTGGCTGGCAGCCCTTTACTGCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605191355_uwqp AAAGCCTTGAGCAGTAAAGGGCTGCCAGCCAG(+CAGTGT)GATCCTCTGGGTCACCGCCGCTTTCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605191353_m9y6 AGGGCATCCTGCCGCGCCGAGGGAGCATCTGG(+CAGTGT)GATCCACACGTCTCCTGTCCCTGCAGACCTG NGG 10 8 17 8 24 0 100 5 10