Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191301_dne5 GCATGGATGTTAACTGAACGCCTGTTGCTGTG(+CAGTGT)GATCCACATACAAGACGCCTTCCTCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605191259_vluj GGAAACTTCCTACGTGTTCGTAGCTACACTGG(+CAGTGT)GTACCAAGGTGCGCGCCGCCGGCGGTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605191257_9pw1 ACGAAGTTGGACCGCCGGCGGCGCGCACCTTG(+CAGTGT)GTACCCAGTGTAGCTACGAACACGTAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605191256_hr8f TAGTAAGCATAAGGGGCTGCCTTCATCTTATG(+CAGTGT)GTACCTTGGACACATCATCCTATGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605191255_p1wu CTGTCCTGTGACCATAGGATGATGTGTCCAAG(+CAGTGT)GTACCATAAGATGAAGGCAGCCCCTTATGCT NGG 10 8 17 8 24 0 100 5 10
    240605191253_tn2i GAGTGTGATCACCAACCACATCCGCTCCTAAG(+CAGTGT)GATCCACATCTTATCCTGCAGGTAACAGACA NGG 10 8 17 8 24 0 100 5 10
    240605191251_tjw6 CACCGTGTCTGTTACCTGCAGGATAAGATGTG(+CAGTGT)GATCCTTAGGAGCGGATGTGGTTGGTGATCA NGG 10 8 17 8 24 0 100 5 10
    240605191249_hbpa AAAAACCCATCAGCCTGAGGCTTTTGCCTTGG(+CAGTGT)GTACCTGCCCATTTTATAGTTAAATAACACT NGG 10 8 17 8 24 0 100 5 10
    240605191248_cka1 ACATTAGTGTTATTTAACTATAAAATGGGCAG(+CAGTGT)GTACCCAAGGCAAAAGCCTCAGGCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605191246_e1wb TGCTTCTGCCAAATGACTAATATGGCCTGCTG(+CAGTGT)GATCCCAAGCACACGAGGGCTTTGAGGTCAC NGG 10 8 17 8 24 0 100 5 10