Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191209_47fs TGTTACATTTATTTACAAAGAGCCTGGTCTAG(+CAGTGT)GATCCATGATACCCAAGTGTCTCCCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240605191207_5t0r CCTTCGAAAACAAAAACTGAAACAAAAGGCTG(+CAGTGT)GATCCTGTGTCCTGGGACCTCTTTGCACTCA NGG 10 8 17 8 24 0 100 5 10
    240605191205_zqw1 GAATGTGAGTGCAAAGAGGTCCCAGGACACAG(+CAGTGT)GATCCAGCCTTTTGTTTCAGTTTTTGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605191204_5hbs TGCTGGCCAAATTCTCCACTATTGAAGGAATG(+CAGTGT)GTACCGATGAAAATGCAAGACACATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605191203_922a CAAGTCAACAAATGTGTCTTGCATTTTCATCG(+CAGTGT)GTACCATTCCTTCAATAGTGGAGAATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605191200_5kpg CCAGCAGCCTGAAAAAATTGAAATGCCCAAGG(+CAGTGT)GATCCACCTTGACTTTTGTTTGTGAGAACAT NGG 10 8 17 8 24 0 100 5 10
    240605191158_e30o TCCTGATGTTCTCACAAACAAAAGTCAAGGTG(+CAGTGT)GATCCCTTGGGCATTTCAATTTTTTCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605191157_ltuu ATGCGAGCCTCTTCCGTGTTTCTGTTATTAAG(+CAGTGT)GTACCTCCACCTGCTGCTCAGAGGCAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605191155_rqkt GGAGCTGTGCTGCCTCTGAGCAGCAGGTGGAG(+CAGTGT)GTACCTTAATAACAGAAACACGGAAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605191154_68o9 AGGTCCCTCCATTCCCCTGTATATGCTAAAAG(+CAGTGT)GTACCATGTTTCCAGGGCTTATCATGTATTT NGG 10 8 17 8 24 0 100 5 10