Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191152_tdvm AATCTAAATACATGATAAGCCCTGGAAACATG(+CAGTGT)GTACCTTTTAGCATATACAGGGGAATGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605191151_um8u TGACAGAGCCTCTCAGGAGACAGCTGTATCAG(+CAGTGT)GATCCTGTCAGCAAGTACTTCTTGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240605191149_p362 TATTGCTGATGCCAAGAAGTACTTGCTGACAG(+CAGTGT)GATCCTGATACAGCTGTCTCCTGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605191148_avcb ACAAAGTAAATGGGGGAGATCTCAGCATAGTG(+CAGTGT)GTACCCACCTTTAATCCAAGTACTTGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605191146_105x TTCTGCTTCCCAAGTACTTGGATTAAAGGTGG(+CAGTGT)GTACCACTATGCTGAGATCTCCCCCATTTAC NGG 10 8 17 8 24 0 100 5 10
    240605191145_332f AATATTGTTTGCTGTTTTATGCTAAAGTTGTG(+CAGTGT)GATCCCTAAAGCTACATAAACCTTATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605191143_719w AAAATTATAAATAAGGTTTATGTAGCTTTAGG(+CAGTGT)GATCCACAACTTTAGCATAAAACAGCAAACA NGG 10 8 17 8 24 0 100 5 10
    240605191142_nvso CAAACATACAGTCTACTCATTCCATAAAAAAG(+CAGTGT)GTACCTTTCCTTCCTTTCCTTTTACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240605191141_cofd GAAAGGGATGAGTAAAAGGAAAGGAAGGAAAG(+CAGTGT)GTACCTTTTTTATGGAATGAGTAGACTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605191140_sg53 ACTGAATGGCAGCTGTGGACAGGATCTATGTG(+CAGTGT)GTACCCTAAATGAATAAGTGAGATACTACCC NGG 10 8 17 8 24 0 100 5 10