Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191138_6xec ACAGGGGGTAGTATCTCACTTATTCATTTAGG(+CAGTGT)GTACCACATAGATCCTGTCCACAGCTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605191137_8h9w ATGGATGGAAGAAGACGGTGTCAAGGAATGAG(+CAGTGT)GTACCTGACGCTTCTCGGCATCTCTGCGATC NGG 10 8 17 8 24 0 100 5 10
    240605191136_9839 ATGCAGATCGCAGAGATGCCGAGAAGCGTCAG(+CAGTGT)GTACCTCATTCCTTGACACCGTCTTCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605191134_lx5y GTCACCGAGTGTTGGTACTTTATAACTGCATG(+CAGTGT)GTACCCTAGAAATGATCGTTTCATCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605191132_3x4n CATTGAAAGAAGATGAAACGATCATTTCTAGG(+CAGTGT)GTACCATGCAGTTATAAAGTACCAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240605191131_18ow ATAATTCATGGGGGGGGGGGGGCGCGCTAGGG(+CAGTGT)GTACCATGATGGATGTGTGGAGGTCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605191129_1169 AGTCATCCATTGACCTCCACACATCCATCATG(+CAGTGT)GTACCCCTAGCGCGCCCCCCCCCCCCCATGA NGG 10 8 17 8 24 0 100 5 10
    240605191128_nh8y GGAAAGGCAGTGGAGGAACTGGGGAGCTCTGG(+CAGTGT)GTACCCAGAATCCTCGAGGTCTCATCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605191126_sujm CAGGTGTCAAGATGAGACCTCGAGGATTCTGG(+CAGTGT)GTACCCAGAGCTCCCCAGTTCCTCCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605191125_wgg8 TGCCAAGAATCACATAGCTGAGGCTATAGATG(+CAGTGT)GTACCGATGACTCTGTGGAACCGTGGTTACA NGG 10 8 17 8 24 0 100 5 10