Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191036_zj7b TTCTTGCATGTATATGCACACTTCTGTATGTG(+CAGTGT)GTACCAAAGTAGCTCAGAGGAGGATGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605191033_qnld CATCACAAACATGGGAAACTCTGGTCCCAGGG(+CAGTGT)GATCCAGCATCCTCCTCTGAGCTACTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605191032_e4e5 GTGGTACCAAAGTAGCTCAGAGGAGGATGCTG(+CAGTGT)GATCCCCTGGGACCAGAGTTTCCCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605191030_lm1u AGAGGCAGACACAGGGAGCAGCTCCAGGAACG(+CAGTGT)GTACCGTGGAGGATTCTGGCCAGGGCATCCC NGG 10 8 17 8 24 0 100 5 10
    240605191029_ql2l ACCCTGGGATGCCCTGGCCAGAATCCTCCACG(+CAGTGT)GTACCGTTCCTGGAGCTGCTCCCTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605191027_8d8x ATAAATAAAAACTCAAGGAAAATAAATACATG(+CAGTGT)GATCCTATGCGGTTGGAGGGCGCGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605191026_vobk CTACATCCAGCCCGCGCCCTCCAACCGCATAG(+CAGTGT)GATCCATGTATTTATTTTCCTTGAGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605191025_gert AGACAGAATGCTGTCCAAAAGGGACAAACATG(+CAGTGT)GATCCCTAGGGAAGACAACTCCTGAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605191023_2fua CCCTGCAAAGCTCAGGAGTTGTCTTCCCTAGG(+CAGTGT)GATCCATGTTTGTCCCTTTTGGACAGCATTC NGG 10 8 17 8 24 0 100 5 10
    240605191021_tnkm GATCTAAGATGCGCTCCTAGGAGCACAGTGGG(+CAGTGT)GATCCCAGGCTGTCTGGAGTATGGATAGCCA NGG 10 8 17 8 24 0 100 5 10