Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190959_1qdt GCGGGACTTCATGGCCAAAGAGTGTGAACCTG(+CAGTGT)GTACCGAAGTTGTCAGCCCATGGATCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605190957_piaq AAAGGAAAAAAAAAGTGTGGGTGTGATGTCTG(+CAGTGT)GATCCGTGTTTGCCTGCAGTAGCCTGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605190956_tgca GGATGTCTCACAGGCTACTGCAGGCAAACACG(+CAGTGT)GATCCAGACATCACACCCACACTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605190954_zbc2 GGTTTGAGAAGCCCAATCTTGTCGGAGACGGG(+CAGTGT)GATCCTCATTGATGGGAGGACTGAGGCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605190953_hip7 CCACTGCAGGCCTCAGTCCTCCCATCAATGAG(+CAGTGT)GATCCCCGTCTCCGACAAGATTGGGCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605190951_qiuv GTCCTCTACCCCCTGTGATGTTGAGCGTGCTG(+CAGTGT)GATCCTGGGGGCTGTCGGAGGCTGGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605190948_pnw8 CTGGCGCTGAACCAGCCTCCGACAGCCCCCAG(+CAGTGT)GATCCAGCACGCTCAACATCACAGGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605190947_h4y7 AACGGCCTCAACAGGGCCGAGAGGCTGACAGG(+CAGTGT)GTACCTGGAGTCCAGTTACTCACTGGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605190945_s3no ACTCAGACAGCCAGTGAGTAACTGGACTCCAG(+CAGTGT)GTACCCTGTCAGCCTCTCGGCCCTGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605190943_c642 TCTACCCCATGTGGTCTGCCTCTGGTGTGGTG(+CAGTGT)GTACCTGGTCCTTTTTATACCCCTTCCACCT NGG 10 8 17 8 24 0 100 5 10