Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190837_ne0l GTGGTAGGTTTTCATGGTTATGACTACATGTG(+CAGTGT)GTACCAGTTCTGTGTTATTTGTTTCCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605190836_ijti TGGTAGACCAGGAAACAAATAACACAGAACTG(+CAGTGT)GTACCACATGTAGTCATAACCATGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605190831_otn6 TACTTTGCTGTATTCAAGTCTGTTTCTCAGTG(+CAGTGT)GATCCAGTGAACTAAATTCTAACATTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605190830_ktwp AAACTGTTCAAATGTTAGAATTTAGTTCACTG(+CAGTGT)GATCCACTGAGAAACAGACTTGAATACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605190828_c4n3 TTTTTTTTTTCTGAACACCAGGTTCTTGCTTG(+CAGTGT)GATCCAGAATAGCCCATCCACTTAGATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605190827_pfrf ATATACACTATCTAAGTGGATGGGCTATTCTG(+CAGTGT)GATCCAAGCAAGAACCTGGTGTTCAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605190825_v55b CCCTAATGCTGGTCCTTAAGAGTCCCTGGGTG(+CAGTGT)GATCCCAGCCCTAGTGACCTTGTCTTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605190823_ej4y AGCCCATGGGAAGACAAGGTCACTAGGGCTGG(+CAGTGT)GATCCACCCAGGGACTCTTAAGGACCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605190821_i0wf CTATCCTTCATATTAGGATGTTGCAAATGTGG(+CAGTGT)GTACCTTCCCCCTTTTCCTTTCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605190820_wztu CTCATCCTCATTCTGAAAGGAAAAGGGGGAAG(+CAGTGT)GTACCCACATTTGCAACATCCTAATATGAAG NGG 10 8 17 8 24 0 100 5 10