Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190732_agy6 CAGGGAAATTGCTACAGATGTTTCACAACCTG(+CAGTGT)GATCCATCCTGTCAGTCAAACAGCAACAACC NGG 10 8 17 8 24 0 100 5 10
    240605190731_0gv3 GAAATGGTTGTTGCTGTTTGACTGACAGGATG(+CAGTGT)GATCCAGGTTGTGAAACATCTGTAGCAATTT NGG 10 8 17 8 24 0 100 5 10
    240605190729_ipch ACTTAAGTTCACACCAGGAGTTAGGCGCTCTG(+CAGTGT)GATCCTGAGCCAGGCAGGCACAAAGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605190728_89jo CTCTAAGGAACCTTTGTGCCTGCCTGGCTCAG(+CAGTGT)GATCCAGAGCGCCTAACTCCTGGTGTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605190727_f379 TCAAATCCTGCAGTAGTGAGTAGCTATGAATG(+CAGTGT)GATCCTATTAACCGGGTGGGAGGGGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605190726_axxe CTGTTTCCACCCCCCTCCCACCCGGTTAATAG(+CAGTGT)GATCCATTCATAGCTACTCACTACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605190724_gtf6 GTGCCATCTCTGAATGCAGAACTCATGTCCAG(+CAGTGT)GTACCAGTACTGTAGTGGAGCATAGGATACA NGG 10 8 17 8 24 0 100 5 10
    240605190723_grdp TGTGCTGTATCCTATGCTCCACTACAGTACTG(+CAGTGT)GTACCTGGACATGAGTTCTGCATTCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605190721_m5o2 GTAATCTCTTCCCTCACATGTAACAAACTCTG(+CAGTGT)GATCCAACCCTCAGTACTACGGATCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605190719_d9xp AGTGAGATTTAGATCCGTAGTACTGAGGGTTG(+CAGTGT)GATCCAGAGTTTGTTACATGTGAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10