Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190626_4zta TAGATGGTCAACTTGATCCTGATTCAACTTTG(+CAGTGT)GTACCTGATATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605190625_g8e1 GACAAATGGACAAATTTCTGGACAGATATCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240605190623_s41t AGTTTAACTTCTGGAGTCCATATAAAGAGCTG(+CAGTGT)GATCCAAAGTGTGCATTTGCAATCCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605190622_nj8p GTTCAGTGCTGGGATTGCAAATGCACACTTTG(+CAGTGT)GATCCAGCTCTTTATATGGACTCCAGAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605190620_snxh ATTGCCCTCATCCACAGAACCGCACCCGCCTG(+CAGTGT)GATCCCACAATGAATTGTGCGGATGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605190618_pypr GGGAGAGCAGACATCCGCACAATTCATTGTGG(+CAGTGT)GATCCAGGCGGGTGCGGTTCTGTGGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605190617_vm07 GATGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGAAGTCTCTACATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605190614_6n4f GGATGGTCCATGTAGAGACTTCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605190613_wqka TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605190611_6mrl TCCTCAGGAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10