Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191755_xhc0 GGAAGAGTTGACCGTGGTTAGATACCCGGAGG(+CAGTGT)GATCCCTGACCTCCGTTTTATAATGGGATAA NGG 10 8 17 8 24 0 100 5 10
    240605191753_bssx GCCAGATTTTCACGGGAAGGTCTTTAGTTCCG(+CAGTGT)GATCCTTGTAAGTATGAGTCCTTCATAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605191752_izrt ATATAACCCTATGAAGGACTCATACTTACAAG(+CAGTGT)GATCCGGAACTAAAGACCTTCCCGTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605191750_p3fj GGATGCTCACCCAACATCTGCTTTCCTGGTTG(+CAGTGT)GATCCAGATTTGCACCATTTAAACATTGATG NGG 10 8 17 8 24 0 100 5 10
    240605191749_kn6p ATGCTCATCAATGTTTAAATGGTGCAAATCTG(+CAGTGT)GATCCAACCAGGAAAGCAGATGTTGGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605191747_ehrg TTCTGCCTCTTTTCATAGTTCTGATGCACACG(+CAGTGT)GTACCGGCTGGTTTTGTGCGTCAACCTGACA NGG 10 8 17 8 24 0 100 5 10
    240605191745_x4t2 GCTTGTGTCAGGTTGACGCACAAAACCAGCCG(+CAGTGT)GTACCGTGTGCATCAGAACTATGAAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605191744_tkxi CTCAGGAATGGGATTGGTGTGATGCCATAATG(+CAGTGT)GTACCTGCCCTGTCCAAGGACAGCCTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605191743_2f3l TGTATGACCCAGGCTGTCCTTGGACAGGGCAG(+CAGTGT)GTACCATTATGGCATCACACCAATCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605191741_akjm GGACACAGTAACCTTGGGTGGTGTGTTCTAGG(+CAGTGT)GTACCTGCCTTCCCGGAAGGCTCAGGGACTA NGG 10 8 17 8 24 0 100 5 10