Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190610_iq7k TTCATTTTCTGCGTGCTCTGACCTTACATTTG(+CAGTGT)GATCCACTCAAAATCTTGCTCCTTGCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605190608_i6sz ATGTGACCAGGCAAGGAGCAAGATTTTGAGTG(+CAGTGT)GATCCAAATGTAAGGTCAGAGCACGCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605190607_o6mk GAGTCATTCTGGGAAAACTAACAATTCATAGG(+CAGTGT)GATCCAGGTCCCTGCCCGGTCCATTAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605190605_r9nm TCCTTTTGTTTAATGGACCGGGCAGGGACCTG(+CAGTGT)GATCCCTATGAATTGTTAGTTTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605190603_st3t ATATAACACTTGGGGGCAGGGGTGGGTGGGGG(+CAGTGT)GTACCTGTATTGTTCAGATTGCTTCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605190601_nrm4 CTTGCTTCTCAGAAGCAATCTGAACAATACAG(+CAGTGT)GTACCCCCCACCCACCCCTGCCCCCAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605190600_4qtb TTTGAAAACAATGAAACAGGATGCTGCTTCTG(+CAGTGT)GTACCCACACCAGCCCCGTTGAACAGATGAT NGG 10 8 17 8 24 0 100 5 10
    240605190558_q4bs GGCCTATCATCTGTTCAACGGGGCTGGTGTGG(+CAGTGT)GTACCAGAAGCAGCATCCTGTTTCATTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605190556_b9e1 TAGTCTCTTGTTCCTTCATAATTGTTCCTTGG(+CAGTGT)GATCCACAACATAGAATTAATCACAGTCGTG NGG 10 8 17 8 24 0 100 5 10
    240605190555_l324 TAAACCACGACTGTGATTAATTCTATGTTGTG(+CAGTGT)GATCCCAAGGAACAATTATGAAGGAACAAGA NGG 10 8 17 8 24 0 100 5 10