Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190718_wboj TTTCGGGGTCTCTGTTTCTTTGTCTATAAAAG(+CAGTGT)GATCCTTTACTAGGATAGAAAGTTGTGATAT NGG 10 8 17 8 24 0 100 5 10
    240605190717_vp7a AAAACATATCACAACTTTCTATCCTAGTAAAG(+CAGTGT)GATCCTTTTATAGACAAAGAAACAGAGACCC NGG 10 8 17 8 24 0 100 5 10
    240605190715_6t2s GGAAGGCCATGCTGTCCACCCTTCCTGGCCAG(+CAGTGT)GATCCAGTTGAGCAGCAGAGGTTGACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605190714_bye3 CCTTCATTTAGTCAACCTCTGCTGCTCAACTG(+CAGTGT)GATCCTGGCCAGGAAGGGTGGACAGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605190712_oo92 TATGGTTAGGTCTTCAGAACTTAATTAAATTG(+CAGTGT)GATCCTGGAGATGAGGCACATGGGTAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605190710_ow1c CTACTTTCTCTACCCATGTGCCTCATCTCCAG(+CAGTGT)GATCCAATTTAATTAAGTTCTGAAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240605190709_k7jc GCTACCCTTAACTTTCACAGATCTAATAATCG(+CAGTGT)GATCCCTAAAATAAACTTGAGGCACATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605190708_xwxv ATGTTCAAAATGTGCCTCAAGTTTATTTTAGG(+CAGTGT)GATCCGATTATTAGATCTGTGAAAGTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605190706_lvjw CAAACTGCTGCCACTCTACAAGCAAATGCCTG(+CAGTGT)GATCCCTTCTGATGTGCAGTAACCAGGACTC NGG 10 8 17 8 24 0 100 5 10
    240605190704_h660 AAGGTGAGTCCTGGTTACTGCACATCAGAAGG(+CAGTGT)GATCCAGGCATTTGCTTGTAGAGTGGCAGCA NGG 10 8 17 8 24 0 100 5 10