Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190802_4k3g AATAAAAGCAGTAAATGCCTGTTAGTAGCAAG(+CAGTGT)GATCCTCAGTAAAAGTGAGCGGGTGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605190801_7qk9 GGCTACAGAGCCACCCGCTCACTTTTACTGAG(+CAGTGT)GATCCTTGCTACTAACAGGCATTTACTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605190800_rc2g TCAATGAAATCTCACATGCTCATTTTACTCAG(+CAGTGT)GATCCAGTAAGAATGCAAAGGGGTTGCTAAG NGG 10 8 17 8 24 0 100 5 10
    240605190759_djen TTTACCTTAGCAACCCCTTTGCATTCTTACTG(+CAGTGT)GATCCTGAGTAAAATGAGCATGTGAGATTTC NGG 10 8 17 8 24 0 100 5 10
    240605190757_p592 CAAACAATTGTGAGCTGCCATGTAGATGCTAG(+CAGTGT)GATCCCTGGTCCTCTAGAAGAGCCATCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605190755_yo2q GGCTAGAGAGATGGCTCTTCTAGAGGACCAGG(+CAGTGT)GATCCTAGCATCTACATGGCAGCTCACAATT NGG 10 8 17 8 24 0 100 5 10
    240605190754_74jr CCTGCCCCATCCTAGCACCCCCAGAGATTTAG(+CAGTGT)GTACCAAGAGTAAGGCAGCCCAAGCCAATAT NGG 10 8 17 8 24 0 100 5 10
    240605190753_jxgq GTCACATATTGGCTTGGGCTGCCTTACTCTTG(+CAGTGT)GTACCTAAATCTCTGGGGGTGCTAGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605190751_35j1 TGGCCTAGACCTTTATATGCTGATCAGGTTGG(+CAGTGT)GTACCAACTGTCAGCCTGTCTGCTGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605190749_k9gr GAGATGGGAGACAGCAGACAGGCTGACAGTTG(+CAGTGT)GTACCCAACCTGATCAGCATATAAAGGTCTA NGG 10 8 17 8 24 0 100 5 10