Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192147_4wcj GACACAATAATCTGACTTCCACTTGAGATTTG(+CAGTGT)GTACCTGAACTGTGGTCCTTGTGCATAATCT NGG 10 8 17 8 24 0 100 5 10
    240605192146_cp9p ATCTATAGTCCTAGGGCTAGGGAGACAGAGAG(+CAGTGT)GATCCCTGGAGCATGGTGGCAGATGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605192143_nk86 TCATTACTCTTCATCTGCCACCATGCTCCAGG(+CAGTGT)GATCCTCTCTGTCTCCCTAGCCCTAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240605192141_bvjp GAGCTGCAGGGAGCCACTCTGAGTCTGTGAGG(+CAGTGT)GATCCACAGAGCCTAAGATGTGCACGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605192140_cy0o GAATTTCAGGCGTGCACATCTTAGGCTCTGTG(+CAGTGT)GATCCCTCACAGACTCAGAGTGGCTCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605192138_hvcx GAAACCAGTGGCTCTGGTACTCAGTAGTTAGG(+CAGTGT)GTACCCTGTGCTTCTTGGTGCTTTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605192137_6oab TTCCAGAAAGGAAAAGCACCAAGAAGCACAGG(+CAGTGT)GTACCCTAACTACTGAGTACCAGAGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605192135_w7q9 TCCCACACTATTAGCTGCTATCAGAAAAACAG(+CAGTGT)GATCCTACAAGTCAAGGTTACTATGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605192134_8wum TAATTAGGAAACATAGTAACCTTGACTTGTAG(+CAGTGT)GATCCTGTTTTTCTGATAGCAGCTAATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605192131_1k2u ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10