Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190941_fyea AGCCAAGGTGGAAGGGGTATAAAAAGGACCAG(+CAGTGT)GTACCACCACACCAGAGGCAGACCACATGGG NGG 10 8 17 8 24 0 100 5 10
    240605190939_d3zu CCTTCTGCACTCAGCCTTTCCTTGGGGCTAGG(+CAGTGT)GATCCATCACATGGGGTAAGGCCTAGCCTAA NGG 10 8 17 8 24 0 100 5 10
    240605190937_4d2o TTGGATTAGGCTAGGCCTTACCCCATGTGATG(+CAGTGT)GATCCCTAGCCCCAAGGAAAGGCTGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605190936_phay GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+CAGTGT)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605190934_f8i4 TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+CAGTGT)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240605190933_28du TTTTCTAAATATCATCAGTCCACATTTGGTTG(+CAGTGT)GATCCAGACATCCAGAGCTGGGGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605190931_pv47 CAGCCCTCTGCACCCCCAGCTCTGGATGTCTG(+CAGTGT)GATCCAACCAAATGTGGACTGATGATATTTA NGG 10 8 17 8 24 0 100 5 10
    240605190930_r3hw GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+CAGTGT)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605190928_5gew TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+CAGTGT)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240605190926_dpz2 GGGGCATGCAGCTGTGTGCTCGCAGCCCTGTG(+CAGTGT)GTACCTTGAGGGTGGGCAGGAGGACGGGACC NGG 10 8 17 8 24 0 100 5 10