Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190925_blyw AGTCAGGTCCCGTCCTCCTGCCCACCCTCAAG(+CAGTGT)GTACCACAGGGCTGCGAGCACACAGCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605190923_vq23 CTGTGAAAGGTGCCAGCTGCCAGCTGAGAAGG(+CAGTGT)GTACCCAGGGGCTGTGGCACACCAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605190920_r64b GGGGAACAGTCCTGGTGTGCCACAGCCCCTGG(+CAGTGT)GTACCCTTCTCAGCTGGCAGCTGGCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605190919_0lhb CAGGGTCTGGTCCAAGCTGGCCTGGCATTCTG(+CAGTGT)GATCCTCCTGCCCCAGCCTCCCCAGTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605190917_p6i0 TGGTAATGGCACTGGGGAGGCTGGGGCAGGAG(+CAGTGT)GATCCAGAATGCCAGGCCAGCTTGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240605190915_n7tj CAGAGCAAACATGATGGCCTGCCATCTCCTGG(+CAGTGT)GATCCCCCAAGGGTGACTTCCACTCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605190912_cev9 GAAAACCAGAGGAGTGGAAGTCACCCTTGGGG(+CAGTGT)GATCCCAGGAGATGGCAGGCCATCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605190911_z8me ATTTCCAAGAAGGAAAGGTCGGCCTTTGTGCG(+CAGTGT)GTACCACAGCCTCAGTTTCCTAGTCTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605190909_8aud TTCACCCTGCAGACTAGGAAACTGAGGCTGTG(+CAGTGT)GTACCGCACAAAGGCCGACCTTTCCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605190907_y974 TCTAAAAGATGAAATATCCACTATCCAAAGGG(+CAGTGT)GATCCTCAGAGGGCAACATAGTCAGCGACCA NGG 10 8 17 8 24 0 100 5 10