Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191018_x9yl CCCCATGGCTATCCATACTCCAGACAGCCTGG(+CAGTGT)GATCCCCACTGTGCTCCTAGGAGCGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240605191016_2wg9 TAGGAGCACTGGCTCTCTTGCAGAGGGCCTGG(+CAGTGT)GATCCATTTCCAGCACCCAACGTGGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605191015_8qmh GTTGTGAGCTGCCACGTTGGGTGCTGGAAATG(+CAGTGT)GATCCCAGGCCCTCTGCAAGAGAGCCAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605191013_olkn TGCAGCTAGGCAGGAGTACTATGGGCCAGGAG(+CAGTGT)GATCCCCTGGATACATGGTCCACGGAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605191010_mx9m CATGGTGCCCTCCGTGGACCATGTATCCAGGG(+CAGTGT)GATCCTCCTGGCCCATAGTACTCCTGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605191009_3s3j CTGGCACTCATCCTTCTGAGGCTGGGCCACAG(+CAGTGT)GATCCAGGTCCCCTTAGCTGTCCCAGGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605191006_yulo CTCCTGGGGCCTGGGACAGCTAAGGGGACCTG(+CAGTGT)GATCCTGTGGCCCAGCCTCAGAAGGATGAGT NGG 10 8 17 8 24 0 100 5 10
    240605191004_rgv8 TAAACAGCATAAGCGGATAGATTCTACTTGGG(+CAGTGT)GATCCTGATGTCAAAGTAAGGACACACGGGA NGG 10 8 17 8 24 0 100 5 10
    240605191002_i2cu CACCCTCCCGTGTGTCCTTACTTTGACATCAG(+CAGTGT)GATCCCCAAGTAGAATCTATCCGCTTATGCT NGG 10 8 17 8 24 0 100 5 10
    240605191000_ypdd ATGACCTGCTGATCCATGGGCTGACAACTTCG(+CAGTGT)GTACCAGGTTCACACTCTTTGGCCATGAAGT NGG 10 8 17 8 24 0 100 5 10