Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191123_67lh TTTATTGTAACCACGGTTCCACAGAGTCATCG(+CAGTGT)GTACCATCTATAGCCTCAGCTATGTGATTCT NGG 10 8 17 8 24 0 100 5 10
    240605191122_pch3 TACGATGGAAATGTATCCTGCCACCTAGCTGG(+CAGTGT)GTACCCATATGGACAGCAACCCAAGGACCCA NGG 10 8 17 8 24 0 100 5 10
    240605191120_3k4a TTGTTTGGGTCCTTGGGTTGCTGTCCATATGG(+CAGTGT)GTACCCAGCTAGGTGGCAGGATACATTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605191119_qyes GCACCTTGACAAAATCAGGGTGTCTAGGGCTG(+CAGTGT)GATCCCCTGAAGCTCTAAGGGAAACTCCATT NGG 10 8 17 8 24 0 100 5 10
    240605191117_mak0 ACCAGAATGGAGTTTCCCTTAGAGCTTCAGGG(+CAGTGT)GATCCAGCCCTAGACACCCTGATTTTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605191114_7nh1 CAGGAAAGTAGGATGTGCCCAAGATGCCCATG(+CAGTGT)GATCCTTTGAGAGGCACACAGCCAGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605191113_bz17 CCTGCCCAGGCCTGGCTGTGTGCCTCTCAAAG(+CAGTGT)GATCCATGGGCATCTTGGGCACATCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240605191111_ckm5 GATGTGTCTAGCTAGCTAGCCAGAATGCTCAG(+CAGTGT)GATCCTCTATGACCTTATTAGCTTTAACTGT NGG 10 8 17 8 24 0 100 5 10
    240605191109_eguj AGTTGACAGTTAAAGCTAATAAGGTCATAGAG(+CAGTGT)GATCCTGAGCATTCTGGCTAGCTAGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605191108_6hgs AGTGGCAGGGGTCAGCTTTCTCCATCATGTAG(+CAGTGT)GTACCAGATATCAAACCCAGGTTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10