Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605191225_64pm AACTTTAGTCCCGGGGGAGAGGAAGCTGGTTG(+CAGTGT)GATCCACAGGAGTCCTGGGGCACTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605191224_lhyo AAACCCCTGGTGCTCTCCCAGACCAGAAAAGG(+CAGTGT)GTACCAGATCTCCTGGAACTGGAATTACAAG NGG 10 8 17 8 24 0 100 5 10
    240605191223_q1i9 AACCACTTGTAATTCCAGTTCCAGGAGATCTG(+CAGTGT)GTACCCTTTTCTGGTCTGGGAGAGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240605191221_dro0 AGTGTGCAGACAAACGGGACATGCGATCTGAG(+CAGTGT)GATCCGAAGCCATACTGCTAACTAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605191220_0fs6 CCCTGCAGGGTCTAGTTAGCAGTATGGCTTCG(+CAGTGT)GATCCTCAGATCGCATGTCCCGTTTGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605191218_njmg TCCGGCTCCTGGGTGCCACTACCAGTCCTGTG(+CAGTGT)GTACCCTGTCTATGCCTGGGACCTTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605191216_wrz4 GCACCCAGCAGAAGGTCCCAGGCATAGACAGG(+CAGTGT)GTACCACAGGACTGGTAGTGGCACCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605191215_ztn8 GCTCTCTGGGTGGCTTACGCTGGATTGTAGTG(+CAGTGT)GATCCAGCCCCACTTGGGGACCTGCCATGTA NGG 10 8 17 8 24 0 100 5 10
    240605191213_0x6d AGAGCTACATGGCAGGTCCCCAAGTGGGGCTG(+CAGTGT)GATCCACTACAATCCAGCGTAAGCCACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605191211_ih5d CTTTTAAACATGGGAGACACTTGGGTATCATG(+CAGTGT)GATCCTAGACCAGGCTCTTTGTAAATAAATG NGG 10 8 17 8 24 0 100 5 10