Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605192550_9n46 AGCAATCCCAGAGTCTGTGACAAGCCAGAATG(+CAGTGT)GTACCAGCAGCAGGACGCAGGGTCTGAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605192549_omi9 ACTTCGTACTCAGACCCTGCGTCCTGCTGCTG(+CAGTGT)GTACCATTCTGGCTTGTCACAGACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605192547_gl6f AAGAGCCACAATGATGGAGGGAGAGAATTGGG(+CAGTGT)GATCCAGCATCAAACACCCCCTCACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605192546_onrx TTGCCTTCCTGGTGAGGGGGTGTTTGATGCTG(+CAGTGT)GATCCCCAATTCTCTCCCTCCATCATTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605192544_2pj3 CCAATAATGAAGTCCCAGATATCAGAAAACTG(+CAGTGT)GATCCTGGCCTGAGTAGGGGGTGATACACAG NGG 10 8 17 8 24 0 100 5 10
    240605192543_x7sn CCATTCTGTGTATCACCCCCTACTCAGGCCAG(+CAGTGT)GATCCAGTTTTCTGATATCTGGGACTTCATT NGG 10 8 17 8 24 0 100 5 10
    240605192541_5v7e TCACCTTATTTTTGTAGTCTCTCCCTGAACTG(+CAGTGT)GTACCTCACCCATTCAGCTGTCCAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605192540_rmjx TCTCAAGTTTTCTGGACAGCTGAATGGGTGAG(+CAGTGT)GTACCAGTTCAGGGAGAGACTACAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605192538_gbz9 AGATGGGGTCTGTGATGGATTGTTACACTGTG(+CAGTGT)GATCCCTGCACTTGGCATCTGCACGAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605192536_82wj TGGCCTAGACTCGTGCAGATGCCAAGTGCAGG(+CAGTGT)GATCCACAGTGTAACAATCCATCACAGACCC NGG 10 8 17 8 24 0 100 5 10