Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190702_i87m TGTGCCTCAAAGTTCGGTTGGGGCTACCTTGG(+CAGTGT)GATCCCAAACAGCTAATATGGTCACCAACTG NGG 10 8 17 8 24 0 100 5 10
    240605190700_ou6r ATAGCCAGTTGGTGACCATATTAGCTGTTTGG(+CAGTGT)GATCCCAAGGTAGCCCCAACCGAACTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605190658_u8ga CTCTGGAGACATCTTCAGCCTGAGGAGGCTGG(+CAGTGT)GTACCAAGGAGGAAGAACCCAGCTGGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605190656_8pvf GGTGGTAAAGCCAGCTGGGTTCTTCCTCCTTG(+CAGTGT)GTACCCAGCCTCCTCAGGCTGAAGATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605190652_1nku ATCCCTCAGGCTCTGCTGTGGACTAGTTCCAG(+CAGTGT)GATCCCTGTCACCAAGTATATGAAAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240605190650_g8ri CATCCTCAGGTTTTCATATACTTGGTGACAGG(+CAGTGT)GATCCTGGAACTAGTCCACAGCAGAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605190648_4kx3 GTTTAAAAAAAGAAAAAAAGAAAAAAAAACAG(+CAGTGT)GTACCTGAAATCCCAGCACTTGAGAGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605190647_4z0d CTGGCTCAACCTCTCAAGTGCTGGGATTTCAG(+CAGTGT)GTACCTGTTTTTTTTTCTTTTTTTCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605190645_e793 TGACCAGCCAGCCACCCTAGCCTACTTGTTGG(+CAGTGT)GTACCAGGCCAATGGGAAAGCCTGTCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605190643_xbxp GCTATTTTGAGACAGGCTTTCCCATTGGCCTG(+CAGTGT)GTACCCAACAAGTAGGCTAGGGTGGCTGGCT NGG 10 8 17 8 24 0 100 5 10