Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190504_kwkw AAGAAGGGAGATAGACCTCAAACTAGTTCTTG(+CAGTGT)GTACCTCACCTTCATCCCCCACCCACCCACT NGG 10 8 17 8 24 0 100 5 10
    240605190502_sf6p TCGGGAGTGGGTGGGTGGGGGATGAAGGTGAG(+CAGTGT)GTACCAAGAACTAGTTTGAGGTCTATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605190500_z15i GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605190458_ndgw GGATGGACCATCTAGTGATTGCCATATGCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605190457_rikb TTAGTCATCATGGATTGTCACTCCTTGAGAAG(+CAGTGT)GATCCAGAGGTGTAGATTTGTTGGGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605190455_57m5 GACATACTTCCCCCAACAAATCTACACCTCTG(+CAGTGT)GATCCTTCTCAAGGAGTGACAATCCATGATG NGG 10 8 17 8 24 0 100 5 10
    240605190453_7twd GTGGGATTCTCTCTATGTGTGTCTTCTACATG(+CAGTGT)GATCCAGAGAGCAGTTCCTAATGGAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240605190452_9ka1 GATACATCGTTTCCATTAGGAACTGCTCTCTG(+CAGTGT)GATCCATGTAGAAGACACACATAGAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605190451_yatk GTTGCCCTTTGATGCTGAGATCTTCCGGTATG(+CAGTGT)GATCCTCCGTGAAGGCAGGCTCAGGGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605190449_yo9f TCTCATCTCACCCTGAGCCTGCCTTCACGGAG(+CAGTGT)GATCCATACCGGAAGATCTCAGCATCAAAGG NGG 10 8 17 8 24 0 100 5 10