Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605190414_bx3e ATCATGCTCAGACAGCTTGCTTGAGGATGTTG(+CAGTGT)GATCCCCTGGTTTCTGGGGAGGCAAGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605190412_56td CTCAAACAAGCTTGCCTCCCCAGAAACCAGGG(+CAGTGT)GATCCAACATCCTCAAGCAAGCTGTCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605190410_ywt5 ACTGAGCGCTCAGCTAACTTGGTGTCTGCTAG(+CAGTGT)GATCCTCTGCTGTAAGCTTTACCCTGCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605190409_t7fl CAAAGGAAAGCAGGGTAAAGCTTACAGCAGAG(+CAGTGT)GATCCTAGCAGACACCAAGTTAGCTGAGCGC NGG 10 8 17 8 24 0 100 5 10
    240605190408_c35n CACCACGCCCGGCTGCGTCACTGCTTTCTGAG(+CAGTGT)GTACCATCAAATTGTTTATTACAGTTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605190406_sbk5 CATGACTGCCAACTGTAATAAACAATTTGATG(+CAGTGT)GTACCTCAGAAAGCAGTGACGCAGCCGGGCG NGG 10 8 17 8 24 0 100 5 10
    240605190404_qu6y AAGGATTGGTGTACATCTTACCTTGTGCTGGG(+CAGTGT)GATCCCAAGTTACCACACCCAGCCTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605190402_dgrm TTCCTGTCTGGAGGCTGGGTGTGGTAACTTGG(+CAGTGT)GATCCCCAGCACAAGGTAAGATGTACACCAA NGG 10 8 17 8 24 0 100 5 10
    240605190400_wvb2 GAGTCGGTCGTAGGGACTGCACTGGGTGCTGG(+CAGTGT)GTACCGTCCTGTCTCCCTCCTCACAGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605190359_xuet AGACAAGCTCCTGTGAGGAGGGAGACAGGACG(+CAGTGT)GTACCCAGCACCCAGTGCAGTCCCTACGACC NGG 10 8 17 8 24 0 100 5 10