Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185136_1fnl TAAAGACCACAGTAGAGAAGTAGTTAGTGCTG(+CAGTGT)GTACCATCCACAGCCACTAGGAACTAACCAC NGG 10 8 17 8 24 0 100 5 10
    240605185135_n7mz CATGAGTGGTTAGTTCCTAGTGGCTGTGGATG(+CAGTGT)GTACCAGCACTAACTACTTCTCTACTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605185133_szum CTCAGTGCTGAAAGACTTGATTTTGTAGCTTG(+CAGTGT)GTACCTCAGCGTTGACTTAGGTGCTCCACTA NGG 10 8 17 8 24 0 100 5 10
    240605185132_mlsc TGGGCTAGTGGAGCACCTAAGTCAACGCTGAG(+CAGTGT)GTACCAAGCTACAAAATCAAGTCTTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605185131_7ndy ATGCCCTCTTTCATGGAGCTGTTCGCACTGCG(+CAGTGT)GATCCGATTCCTCGGGCTTGGATTCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240605185130_0qb2 TCTCGGGAGTGGAATCCAAGCCCGAGGAATCG(+CAGTGT)GATCCGCAGTGCGAACAGCTCCATGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605185128_h0vk TCCCTGGCCTGGGCGCCCCTTGCTGTTGGGAG(+CAGTGT)GATCCCATCCTCGAGTGCCTCCTATCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605185126_23a7 AAGGCAGGAGGATAGGAGGCACTCGAGGATGG(+CAGTGT)GATCCTCCCAACAGCAAGGGGCGCCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605185124_n34a TGACTTCTGAAACGAAGAGAAAAGCACCTATG(+CAGTGT)GATCCCACTCCTTCCCATCGCTTACCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605185122_2vy0 CTGGGGAACAGGTAAGCGATGGGAAGGAGTGG(+CAGTGT)GATCCATAGGTGCTTTTCTCTTCGTTTCAGA NGG 10 8 17 8 24 0 100 5 10