Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185039_5e39 TTATCTAGTCTGGGACCCCAGCCTATGAAACG(+CAGTGT)GTACCACCACAGTTAGGGTGGGTCTTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605185038_rmfd GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+CAGTGT)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605185036_heiu CCTACCCCAGTGATATAATTACCCCCAGCAAG(+CAGTGT)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605185035_ax3g GTTTTCACTAGGTATAGAGCAGTAGTCACCAG(+CAGTGT)GTACCAACACAGGGAGCCCTCCACACACTCT NGG 10 8 17 8 24 0 100 5 10
    240605185033_9r52 TCTTCAGAGTGTGTGGAGGGCTCCCTGTGTTG(+CAGTGT)GTACCTGGTGACTACTGCTCTATACCTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605185032_a665 TCAAGAACTCTGTCTTACCATTTCTTGTCTAG(+CAGTGT)GTACCTAGCATGGCAAACCTGTGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605185031_e00z AATGACTCTCTCCCACAGGTTTGCCATGCTAG(+CAGTGT)GTACCTAGACAAGAAATGGTAAGACAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605185029_x9z9 GATTTTGGCCTTTTTCAGTTGGGTACTATATG(+CAGTGT)GTACCAGGATGAATACATGGCCTTTAATGTT NGG 10 8 17 8 24 0 100 5 10
    240605185028_to5l GAAGGAACATTAAAGGCCATGTATTCATCCTG(+CAGTGT)GTACCATATAGTACCCAACTGAAAAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605185026_5viu GCTTGCTTCATAAGCATGAAGACTGCCATTTG(+CAGTGT)GATCCTAGTACCCAAACAAAAGCTGGGCATT NGG 10 8 17 8 24 0 100 5 10