Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184953_hzz3 CAGCGGAGGTGCCCGCAGAACAGGGGAGAGGG(+CAGTGT)GATCCATGAGACCAGTCCTCTCCTGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605184952_eb3j TTCTGTGTGTAGGGGTCTGTCCATGATGTTGG(+CAGTGT)GTACCACCTGAATTAATCCCGGATTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605184950_6tw2 ACACATAACAAAATCCGGGATTAATTCAGGTG(+CAGTGT)GTACCCAACATCATGGACAGACCCCTACACA NGG 10 8 17 8 24 0 100 5 10
    240605184949_bfu1 ACACACAGCATGTAATTAACAACAGATCTATG(+CAGTGT)GATCCAGCTCTTAATATTGATCCTTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605184947_px02 AACACCACCAAAAGGATCAATATTAAGAGCTG(+CAGTGT)GATCCATAGATCTGTTGTTAATTACATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605184946_o18l ATGTAGAGTCTCTTAGAGGACAGCCATACTAG(+CAGTGT)GATCCTATCTGCAAGCACAACATGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240605184945_ec1y TATTACTGATGCCATGTTGTGCTTGCAGATAG(+CAGTGT)GATCCTAGTATGGCTGTCCTCTAAGAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605184943_nbpj ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+CAGTGT)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240605184942_7eh0 AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+CAGTGT)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240605184941_kvy8 ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+CAGTGT)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10