Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184925_43um GAAGCAGCTACTCTATCATGAAGAACAGTTTG(+CAGTGT)GTACCCGCCACCAGAACATGGTTAATCACTT NGG 10 8 17 8 24 0 100 5 10
    240605184924_8ltt TCATAGTGGTCCACTGAGGTCATATATTTCTG(+CAGTGT)GATCCTTCCGTCTTAGGAAATATCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605184922_hm4d CAGAAGAGGGTTGATATTTCCTAAGACGGAAG(+CAGTGT)GATCCAGAAATATATGACCTCAGTGGACCAC NGG 10 8 17 8 24 0 100 5 10
    240605184920_vddu CAGAGTTCCCTGTAGATGGACTGATTCACCAG(+CAGTGT)GATCCTGCTACACTGAAGGCGCACAGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605184919_7p58 GGCTGGTCAACTGTGCGCCTTCAGTGTAGCAG(+CAGTGT)GATCCTGGTGAATCAGTCCATCTACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605184918_2bhi GTGATTAGAGTAGGTGGTCGGGGCCACACTAG(+CAGTGT)GTACCTAACCTACACACAAGCACACAGAATC NGG 10 8 17 8 24 0 100 5 10
    240605184917_kbtn TGCCAGATTCTGTGTGCTTGTGTGTAGGTTAG(+CAGTGT)GTACCTAGTGTGGCCCCGACCACCTACTCTA NGG 10 8 17 8 24 0 100 5 10
    240605184915_pykh GTTCTAAGGGGAAAATATCCTTTGAGAATTGG(+CAGTGT)GTACCATAGTGACAAAGCCTTGACAACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605184914_t4lu CCCATTCCTGTTGTCAAGGCTTTGTCACTATG(+CAGTGT)GTACCCAATTCTCAAAGGATATTTTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605184912_55ae AGGAGGGCCAGGCAGGGAGGGTCCCTAGAGGG(+CAGTGT)GATCCCTCAGCCCTCGAGGCTGCAGCAGCCA NGG 10 8 17 8 24 0 100 5 10