Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184940_7xc7 AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+CAGTGT)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240605184938_cnv7 TTTAGTCACAATAAAGACCCCAAATGAATAAG(+CAGTGT)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240605184937_kqhv TGTGTATATATGTGTATATATATACACACATG(+CAGTGT)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605184936_jd60 TTTAGTCACAATAAAGACCCCAAATGAATAAG(+CAGTGT)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240605184935_3vht TGTGTATATATGTGTATATATATACACACATG(+CAGTGT)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605184933_je1i TTTAGTCACAATAAGGACCCCAAATGAATAAG(+CAGTGT)GTACCATGTGTGTGTGTGTGTGTGTGTATAC NGG 10 8 17 8 24 0 100 5 10
    240605184932_7sqa TATGTGTATACACACACACACACACACACATG(+CAGTGT)GTACCTTATTCATTTGGGGTCCTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605184931_rd5k TAGTGTTTAAAGCATGTGATGCAGTGGCAGAG(+CAGTGT)GATCCAGGTTTAGTTCCTAGCACCTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605184929_80oi AGGTGCCACACAGGTGCTAGGAACTAAACCTG(+CAGTGT)GATCCTCTGCCACTGCATCACATGCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605184927_s0dn GTGGTAAGTGATTAACCATGTTCTGGTGGCGG(+CAGTGT)GTACCAAACTGTTCTTCATGATAGAGTAGCT NGG 10 8 17 8 24 0 100 5 10