Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184853_vv1s CTCCCAAAGGGGAGCTCTAGGGTAGTCAGTGG(+CAGTGT)GTACCAGAGGCCTTGCTCGGCCTCGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605184851_ujwx CCAATTAAATTAAAACCTTAGCTAGGACCCAG(+CAGTGT)GTACCAGTATGTTTATTGGGAGCCACTGATA NGG 10 8 17 8 24 0 100 5 10
    240605184849_6vu2 CTATGTATCAGTGGCTCCCAATAAACATACTG(+CAGTGT)GTACCTGGGTCCTAGCTAAGGTTTTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605184847_7lt5 CTGGAGACCGTGGGGTCTAGGTAGCCTGCCAG(+CAGTGT)GATCCCAGCAGTGAGCTCACTTTGGGCCATT NGG 10 8 17 8 24 0 100 5 10
    240605184845_gjh2 AATGAAATGGCCCAAAGTGAGCTCACTGCTGG(+CAGTGT)GATCCTGGCAGGCTACCTAGACCCCACGGTC NGG 10 8 17 8 24 0 100 5 10
    240605184844_srph AAGGCTGAGATTAAAGAAAGGTTCCTGCTGTG(+CAGTGT)GTACCCAACTTTTTATCTGGGTGCTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605184842_ogn5 CACGGGGACACAGCACCCAGATAAAAAGTTGG(+CAGTGT)GTACCACAGCAGGAACCTTTCTTTAATCTCA NGG 10 8 17 8 24 0 100 5 10
    240605184840_b1ms CATGTAGAACACAAGAAGACCCTTTTCTTCTG(+CAGTGT)GATCCTCTGCTAGCTACCATCTAAACTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605184839_syvl AAATGGGACAGTTTAGATGGTAGCTAGCAGAG(+CAGTGT)GATCCAGAAGAAAAGGGTCTTCTTGTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605184837_c1tf TTGGCTAAACTGACTGGCATGCGAGCACCAGG(+CAGTGT)GATCCTCCTGTCTCTGCCTTCCAAAGGCTGA NGG 10 8 17 8 24 0 100 5 10