Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184802_x9da ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605184801_5swc GAATCCCTTTTCCTACATGTCCTGGGAAGCTG(+CAGTGT)GATCCCTGGCCTTCCCACCTTCCCACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605184758_kury GGGTGAGGGGGTGGGAAGGTGGGAAGGCCAGG(+CAGTGT)GATCCAGCTTCCCAGGACATGTAGGAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605184757_chx8 TTAAGTGGATTGACAATGACAGAACATGTCTG(+CAGTGT)GATCCATCCTTAGACATGCTTTTGCTGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605184755_hi3i TATCAACTTCAGCAAAAGCATGTCTAAGGATG(+CAGTGT)GATCCAGACATGTTCTGTCATTGTCAATCCA NGG 10 8 17 8 24 0 100 5 10
    240605184754_6sdz GGGCCACTTCCTGTCCACTTGACAGCCTGCAG(+CAGTGT)GTACCGCACTGGCAAAGCTTTGGCCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605184753_xv4i CCCATCTCTGGGGCCAAAGCTTTGCCAGTGCG(+CAGTGT)GTACCTGCAGGCTGTCAAGTGGACAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605184751_buuo GAGGGAGGGGGTATATGTAGCAAGTGTGCTTG(+CAGTGT)GATCCTGGTTAGGGCAGGGCCACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605184749_727t AAGTGGACAGGAAGTGGCCCTGCCCTAACCAG(+CAGTGT)GATCCAAGCACACTTGCTACATATACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605184748_8ypb GGTTGGGGGCTCTGCCATCCCTCCCCTGCTGG(+CAGTGT)GTACCCTGTTACCATTCATTGTCTGTGCTGT NGG 10 8 17 8 24 0 100 5 10