Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184712_4w3w AATCAAGCTCTCCCAATAAACATGTGCAGAAG(+CAGTGT)GATCCTGTTGCAGCGTCGTTACTGCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605184710_aasd AACAATGCTAGCCTGGCCTTCTGGGTCCATGG(+CAGTGT)GTACCAGCTCTGGCACCGCCATCCCTGATCT NGG 10 8 17 8 24 0 100 5 10
    240605184709_fq78 CACTGAGATCAGGGATGGCGGTGCCAGAGCTG(+CAGTGT)GTACCCATGGACCCAGAAGGCCAGGCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240605184707_inah AGCACAGACACTCGTGTCTCACATCACACTGG(+CAGTGT)GTACCTAGGGGCGGATGTGGACACACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605184704_gt6g ATCTCCTTGGGTGTGTCCACATCCGCCCCTAG(+CAGTGT)GTACCCAGTGTGATGTGAGACACGAGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605184703_7jkz AATCGAGGGAAAAAATTACATATGACTGCTTG(+CAGTGT)GTACCTCTTCATTTGGCAAAGGCAGCCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605184702_rn9b TAGTCTTGAGGCTGCCTTTGCCAAATGAAGAG(+CAGTGT)GTACCAAGCAGTCATATGTAATTTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605184701_djjf GCAGCCGAGCATGAGCGTTACGGGAAGAGATG(+CAGTGT)GATCCTTACCAGGTTGTGAGGCGGGAACGAC NGG 10 8 17 8 24 0 100 5 10
    240605184659_lim6 GAGCAGTCGTTCCCGCCTCACAACCTGGTAAG(+CAGTGT)GATCCATCTCTTCCCGTAACGCTCATGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605184658_0r96 TTTTTTCCCCCACGATAGCATGTTTATTTTAG(+CAGTGT)GATCCATTAAATCATACATCTTTTCAAGCTA NGG 10 8 17 8 24 0 100 5 10