Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184620_qcfc CCTCACAGGCGCCTGGCTGTGTCTGCCCTGAG(+CAGTGT)GTACCCTTGCCTCTCCTGCCTGATGAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605184618_2d54 CAGACTGCCCTTGGCAGCAACCCACCCTAGGG(+CAGTGT)GATCCCGTTCCTGACAAACTCCAGCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605184617_5xki AGGTCTCTGGGGCTGGAGTTTGTCAGGAACGG(+CAGTGT)GATCCCCTAGGGTGGGTTGCTGCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605184615_t36q CGGCCAACCTTACCTCATTACTGTGAAGCGAG(+CAGTGT)GATCCTTGTTTGCACACGCTTTGACTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605184614_khan TGTTAAATCCAGTCAAAGCGTGTGCAAACAAG(+CAGTGT)GATCCTCGCTTCACAGTAATGAGGTAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605184613_4noz AAAGAAGAAGCTGTAAGGAGAGTTGCCACTTG(+CAGTGT)GTACCACCCCAGGCTGGACCCCTACAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605184611_5wir CCCCGGCTGCTGTAGGGGTCCAGCCTGGGGTG(+CAGTGT)GTACCAAGTGGCAACTCTCCTTACAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605184610_7pst GGACGTTTGAACCTCAGTTCCAGCGGAGCATG(+CAGTGT)GTACCAAGTCTCAATTCCCATCCTTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605184608_3x7v GGTTCACTGGGAAGGATGGGAATTGAGACTTG(+CAGTGT)GTACCATGCTCCGCTGGAACTGAGGTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605184607_5bq9 AGTAGCTTAAAACAGAGGTTAAGAGCAAAATG(+CAGTGT)GTACCTCAGACACTATACTATTTGTAACTTG NGG 10 8 17 8 24 0 100 5 10