Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184550_928x GTTCCTACAGCGCTCGTTCTAGAACACAAGTG(+CAGTGT)GATCCTTTTTGAATGTTCATGGAAGGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605184549_xpf4 CTTATAGCTGTTGAGTATTTTCAATATCACTG(+CAGTGT)GATCCCTCAGTCTTCACCCATTGGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240605184547_3o4o CTTATATATTTACCAATGGGTGAAGACTGAGG(+CAGTGT)GATCCAGTGATATTGAAAATACTCAACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605184546_l6l3 CAGTATACTATGATCAAAGTGAGACGATAAAG(+CAGTGT)GATCCTGCAATCTAAAGAGACAGAAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240605184544_1g90 TTCACTGTTACTTCTGTCTCTTTAGATTGCAG(+CAGTGT)GATCCTTTATCGTCTCACTTTGATCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240605184543_32dh TGGGAGTGTTTCCTGCCATGCATGAAGCCTTG(+CAGTGT)GATCCACCCCAGCGTTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605184540_o9fz CTCCCACACCCAGTTCACACAACGCTGGGGTG(+CAGTGT)GATCCAAGGCTTCATGCATGGCAGGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605184538_68aw AGACCTGCCTGCCTCTGCCTCCCAAGTGCTGG(+CAGTGT)GATCCAAGGTGTGAGCCACAGCCTGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605184536_3ucm AGGCCTGAAGCCAGGCTGTGGCTCACACCTTG(+CAGTGT)GATCCCAGCACTTGGGAGGCAGAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605184534_f85v ACGAGAAATGTGGGTTCATTTAGTTTAATAGG(+CAGTGT)GATCCATGATTGAGGACTAGGGTGAGTAGTT NGG 10 8 17 8 24 0 100 5 10