Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605185203_tmor ACCAATGTGCAGTCTACGTACATAATCTATGG(+CAGTGT)GATCCCATAACCTGATCTGGCCTGGAGACCA NGG 10 8 17 8 24 0 100 5 10
    240605185202_zpai AGACACTGCTCATGTGACCTTCTACCTTTGTG(+CAGTGT)GATCCCTCTTGGTTTGTGACCTTCGTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605185200_jxvt CAGGGAAGGTACGAAGGTCACAAACCAAGAGG(+CAGTGT)GATCCACAAAGGTAGAAGGTCACATGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605185158_y9w8 TAGCAGCTCACAACTGTAACTTCAATTCTGGG(+CAGTGT)GATCCCATGTCTTTTTCTGACCTCCACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605185156_mbx2 TAAGTCCCTGTGGAGGTCAGAAAAAGACATGG(+CAGTGT)GATCCCCAGAATTGAAGTTACAGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605185154_941m CAACATATGTAAAGCCGTGGGTTGAACCTATG(+CAGTGT)GATCCCTCAGCCTCCAAAAGAGGCAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605185153_wyx2 ACCAAAGCATCTGCCTCTTTTGGAGGCTGAGG(+CAGTGT)GATCCATAGGTTCAACCCACGGCTTTACATA NGG 10 8 17 8 24 0 100 5 10
    240605185151_s9r4 AGTGTCTGGGTTTGATGGCTGATTATGGGATG(+CAGTGT)GATCCCCGGGTGGGGTAGTCTCTGGAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605185148_frfb GGATGGACTTTCCAGAGACTACCCCACCCGGG(+CAGTGT)GATCCATCCCATAATCAGCCATCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605185147_ot6e AAACTGTCCAAAGCACTGGACTGACATCACTG(+CAGTGT)GTACCAGGGACGCCTACCTTTCTGGGGTATT NGG 10 8 17 8 24 0 100 5 10