Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184443_z39e AAAAAAAAAAGTCCTGGTCTAATGATTATCTG(+CAGTGT)GATCCAGCCTAATTCTCAGATTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605184441_e6f5 TAGTGATTCTTGAGAATCTGAGAATTAGGCTG(+CAGTGT)GATCCAGATAATCATTAGACCAGGACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605184439_9dbu AAGGTAGATGCCAAACACCAGCATCTCACTGG(+CAGTGT)GATCCACAATCAGGAAATACTTCAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240605184438_wu6e CCTGGCCAGTGCTGAAGTATTTCCTGATTGTG(+CAGTGT)GATCCCAGTGAGATGCTGGTGTTTGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240605184437_hy7m TTTGCAGGAGTTGGTTCTCCTTGAACTGAGTG(+CAGTGT)GATCCTAGGGATTAAACGCCTGTCACTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605184434_gsvp CCTCAACACAGTGACAGGCGTTTAATCCCTAG(+CAGTGT)GATCCACTCAGTTCAAGGAGAACCAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240605184433_rgas GACGGGCACGGTGAGCTAGGACAAGGAACGAG(+CAGTGT)GATCCAGGAGTCTGTGTAGGAATTAGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605184431_xg6r CACCCATCTTCTAATTCCTACACAGACTCCTG(+CAGTGT)GATCCTCGTTCCTTGTCCTAGCTCACCGTGC NGG 10 8 17 8 24 0 100 5 10
    240605184429_2cro GAGAAGAAGGAGGAGGTACAGGGTGCTTTTTG(+CAGTGT)GTACCAGGGAGAAAGTCATTGTGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605184427_75ba AACTACCTCTTCCCACAATGACTTTCTCCCTG(+CAGTGT)GTACCAAAAAGCACCCTGTACCTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10