Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184516_7gma TTCTCCTGCCTCTTTCTGAGTGCTGGAATAAG(+CAGTGT)GTACCTACCTTAAAAACTCAGATAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240605184515_fmrl CACCCACACCTGTCTGTACCTCACTCCAGAGG(+CAGTGT)GATCCACCGCCCTCTTCTGCCCTCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605184513_v6aq CAGGTGCCTGCAGAGGGCAGAAGAGGGCGGTG(+CAGTGT)GATCCCTCTGGAGTGAGGTACAGACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605184510_vd9d CAGCTTTCCTCTCTTTACCTTTCACGCCTTGG(+CAGTGT)GATCCTAAGGATAAAGCCCTGGTTGGTAGTA NGG 10 8 17 8 24 0 100 5 10
    240605184508_0fzp TTAGTTACTACCAACCAGGGCTTTATCCTTAG(+CAGTGT)GATCCCAAGGCGTGAAAGGTAAAGAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605184507_3hrw AAAACGGCAGCACCTCACGGCTGTCTTTCTAG(+CAGTGT)GATCCTCCATTATTCCTTAGAAGCAAGGACC NGG 10 8 17 8 24 0 100 5 10
    240605184506_kkha GACCAGGTCCTTGCTTCTAAGGAATAATGGAG(+CAGTGT)GATCCTAGAAAGACAGCCGTGAGGTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605184504_29sb GGCCCGTGTAGTTGGCTCAGTGTCACACAACG(+CAGTGT)GTACCAGAGCACCATGATGCCTTTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605184503_ubj0 GCGGTCAGACAGAAAGGCATCATGGTGCTCTG(+CAGTGT)GTACCGTTGTGTGACACTGAGCCAACTACAC NGG 10 8 17 8 24 0 100 5 10
    240605184501_z8gv CACCCTTGGGCCTACCTGTTTGCCTGAGGTGG(+CAGTGT)GATCCCAGGAGCCTCACCCCTACCACCCCTC NGG 10 8 17 8 24 0 100 5 10