Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184606_s88v GTAGCCAAGTTACAAATAGTATAGTGTCTGAG(+CAGTGT)GTACCATTTTGCTCTTAACCTCTGTTTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605184605_807m AAAATGATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCACTTAATTGG NGG 10 8 17 8 24 0 100 5 10
    240605184603_eocw TGTGACCAATTAAGTGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605184602_7oc2 ATGAAATGGACAACTTTCTAGACAGATACCAG(+CAGTGT)GTACCAAAGTTAAATCAAGATTAGGTTAATA NGG 10 8 17 8 24 0 100 5 10
    240605184600_et9p TAGATTATTAACCTAATCTTGATTTAACTTTG(+CAGTGT)GTACCTGGTATCTGTCTAGAAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605184558_d4nd TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605184556_o9bp TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605184554_6isg GGATGGACCATCTAGTGATTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605184553_i0xi GTGTCAGCCTTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605184551_vg2g AACTCCTGGCCCTTCCATGAACATTCAAAAAG(+CAGTGT)GATCCACTTGTGTTCTAGAACGAGCGCTGTA NGG 10 8 17 8 24 0 100 5 10