Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184407_skdo GTCTTGTCTCAGCCCCCCTCAAAACAGTGAAG(+CAGTGT)GTACCCAATGTTTATCTGCCCCAGACACCGT NGG 10 8 17 8 24 0 100 5 10
    240605184406_1evk TGGAAACGGTGTCTGGGGCAGATAAACATTGG(+CAGTGT)GTACCTTCACTGTTTTGAGGGGGGCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605184402_q9rv ACACAGCCTTATGAGGAGATGCCCTGCCATGG(+CAGTGT)GATCCGTATTAGCATCCCAGTCCTTCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605184401_d3y4 GAAAGCTGAAGAAGGACTGGGATGCTAATACG(+CAGTGT)GATCCCATGGCAGGGCATCTCCTCATAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605184400_9s9o ACGTTTGTCCAAGAATACAGTGCTTTTTGCTG(+CAGTGT)GATCCAGGTACTGGGTATTAACAGTCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605184358_9ft9 CCCCCTTCAGGACTGTTAATACCCAGTACCTG(+CAGTGT)GATCCAGCAAAAAGCACTGTATTCTTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605184356_8kcr CTTTTAGTTAGGGAACAGCAGCTTTACCCTCG(+CAGTGT)GATCCTGTCAGGCTCCTTTTCCAGATGACGC NGG 10 8 17 8 24 0 100 5 10
    240605184355_wr4w GTGTGGCGTCATCTGGAAAAGGAGCCTGACAG(+CAGTGT)GATCCGAGGGTAAAGCTGCTGTTCCCTAACT NGG 10 8 17 8 24 0 100 5 10
    240605184353_7vsh GTGAGTAGTAGGAACGAGATTCTTCATCCATG(+CAGTGT)GTACCCTTATCTAATTATGGGATTCGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605184351_100l CCAGCCAGAGCGAATCCCATAATTAGATAAGG(+CAGTGT)GTACCATGGATGAAGAATCTCGTTCCTACTA NGG 10 8 17 8 24 0 100 5 10