Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184257_w6tc TGTCCGTTTCCCCTCACTGTCAGGGAAAGCTG(+CAGTGT)GATCCCTCTGGCTGACTTAGAGTACTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605184256_lz2p CCCAAGTCACAGTACTCTAAGTCAGCCAGAGG(+CAGTGT)GATCCAGCTTTCCCTGACAGTGAGGGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605184254_m11r GCCGCTGGCTGGGTGGCCCAGGGGGGCTGGCG(+CAGTGT)GTACCGCCATGCTGGTTCCTTGGGGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605184252_16at TTCCAGAGGCCCCCCAAGGAACCAGCATGGCG(+CAGTGT)GTACCGCCAGCCCCCCTGGGCCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605184250_8kka GAGCCAGAGTTGGGGATCTGAGCCCATGAATG(+CAGTGT)GTACCGCTTAGACCTCAGGCCAAAACACACC NGG 10 8 17 8 24 0 100 5 10
    240605184249_0aqg GGGAGGGTGTGTTTTGGCCTGAGGTCTAAGCG(+CAGTGT)GTACCATTCATGGGCTCAGATCCCCAACTCT NGG 10 8 17 8 24 0 100 5 10
    240605184248_csf8 CCAATCAGACCTAGATCCTGGCTGCTCTAGTG(+CAGTGT)GTACCAAGGTGTCCTGAGATATGGATGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605184246_77kc TGCACACAACATCCATATCTCAGGACACCTTG(+CAGTGT)GTACCACTAGAGCAGCCAGGATCTAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605184245_gif3 GTGTTATATGTTAAAACTTTCTCTTTAGCAAG(+CAGTGT)GTACCAAGAAATTCACCTGTTCCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240605184244_6juh GCCCATATCTGAAGGAACAGGTGAATTTCTTG(+CAGTGT)GTACCTTGCTAAAGAGAAAGTTTTAACATAT NGG 10 8 17 8 24 0 100 5 10