Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184228_zvqx CCAGGCCACAAACCTGCCTGTGGGGCACTGAG(+CAGTGT)GATCCTAGAGGATGCCATCTTCAGGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605184226_jxac CTCCTCCCCTACCTGAAGATGGCATCCTCTAG(+CAGTGT)GATCCTCAGTGCCCCACAGGCAGGTTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605184225_7u2w GGATGGAACTTGAGAATATCATCCTGAGTGAG(+CAGTGT)GTACCCCGGAGCCAGCAAGACAGATGATATA NGG 10 8 17 8 24 0 100 5 10
    240605184222_3n6x GAGTATATATCATCTGTCTTGCTGGCTCCGGG(+CAGTGT)GTACCTCACTCAGGATGATATTCTCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605184220_ia6z AACTAAGCCACTGAAATCGAATTGAATCAGGG(+CAGTGT)GATCCCAAATAAAAAGATCATATTAAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605184219_wy1h AGAATTTGCCTTAATATGATCTTTTTATTTGG(+CAGTGT)GATCCCCTGATTCAATTCGATTTCAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605184216_qnp7 TGGATCCCACAAGGGACCACCAAGTGACCTGG(+CAGTGT)GTACCTCAGTCATGTTAAAACATGTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605184215_0560 AGGAAAGGAAAACATGTTTTAACATGACTGAG(+CAGTGT)GTACCCAGGTCACTTGGTGGTCCCTTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605184213_cw1f TTCATTTATGATTGGCTAACAAGTAACACCTG(+CAGTGT)GATCCCACAAGGGACCACCAAGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605184211_d70b AGGTACCCAGGTCACTTGGTGGTCCCTTGTGG(+CAGTGT)GATCCAGGTGTTACTTGTTAGCCAATCATAA NGG 10 8 17 8 24 0 100 5 10