Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184137_gxv2 ACTAGATATCCACATATTAGAGAATGAAACTG(+CAGTGT)GATCCCTAAAATGTACCACATGCAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605184136_nrwr TTGATTCATTTTTGCATGTGGTACATTTTAGG(+CAGTGT)GATCCAGTTTCATTCTCTAATATGTGGATAT NGG 10 8 17 8 24 0 100 5 10
    240605184134_i0z7 TTAGAACTTTTAAAAAATAAACTTTCTTTTTG(+CAGTGT)GTACCTCTGGCTTCCTATATATGTCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605184132_v18e AATGTTTCCAAGACATATATAGGAAGCCAGAG(+CAGTGT)GTACCAAAAAGAAAGTTTATTTTTTAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605184130_mrce GGTAACTTCATTACAGTGTCTATAAAGTTGTG(+CAGTGT)GATCCCAGTCTTGGTGCCAAATGCATATAAA NGG 10 8 17 8 24 0 100 5 10
    240605184129_6x0b AGCTTTTTATATGCATTTGGCACCAAGACTGG(+CAGTGT)GATCCACAACTTTATAGACACTGTAATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605184128_hm93 AGGATTCATAGTTGATCATCTTCCAGTTGATG(+CAGTGT)GTACCAACTAGATCTTATAAGTACTAGATAT NGG 10 8 17 8 24 0 100 5 10
    240605184126_u2o4 TGTGGATATCTAGTACTTATAAGATCTAGTTG(+CAGTGT)GTACCATCAACTGGAAGATGATCAACTATGA NGG 10 8 17 8 24 0 100 5 10
    240605184124_mha8 TGGGTGCCCATAGAGGTTACAAGGGGCCATTG(+CAGTGT)GATCCTTTTGACCTGGAGTTGAGGAATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605184123_33vf AAAACTAACATTCCTCAACTCCAGGTCAAAAG(+CAGTGT)GATCCAATGGCCCCTTGTAACCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10