Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605184048_z3ua TAAGGAACAACTAGAATTTAGTAACATTTGAG(+CAGTGT)GATCCCAGTGTGGGTCTGAGGAAGGATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605184046_iw1e GCCCTAAGAATCCTTCCTCAGACCCACACTGG(+CAGTGT)GATCCTCAAATGTTACTAAATTCTAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605184045_huvx AACTACTGGGTTGAGATATTTTCTAAAATCTG(+CAGTGT)GATCCCTAAACATCCCAATGTGCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605184043_kcvy AAGTATTTATTCAGCACATTGGGATGTTTAGG(+CAGTGT)GATCCAGATTTTAGAAAATATCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605184041_bsn7 GCTTAGAACCATCTGTAACTCTAGTTCCAGAG(+CAGTGT)GATCCAGTGTTTTCTGGCCTCCATAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605184040_z5pc TGCCTGCTCTCTATGGAGGCCAGAAAACACTG(+CAGTGT)GATCCTCTGGAACTAGAGTTACAGATGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605184039_5hy7 CTTCCCTCCTGACCTCAAGCCATGTAGAGTTG(+CAGTGT)GATCCCAAAAGCCCTGAGCAATGGCACTTTC NGG 10 8 17 8 24 0 100 5 10
    240605184038_e3vw CATAGGAAAGTGCCATTGCTCAGGGCTTTTGG(+CAGTGT)GATCCAACTCTACATGGCTTGAGGTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605184036_4e0y CTGTGGTTCAGGGCTGATCAGAGTCCGGGTGG(+CAGTGT)GATCCTAGCTGTCTGTGATTTGCTGGCTCCG NGG 10 8 17 8 24 0 100 5 10
    240605184035_jnep CAGAGCGGAGCCAGCAAATCACAGACAGCTAG(+CAGTGT)GATCCCACCCGGACTCTGATCAGCCCTGAAC NGG 10 8 17 8 24 0 100 5 10