Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605183940_ymq8 GGAGAGCAGGGGAAAACAGTGGGAAGTGGTTG(+CAGTGT)GTACCCTTCAAAAAGGACTCTTACCCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605183939_n6vc GCGGGGAAAGGGGTAAGAGTCCTTTTTGAAGG(+CAGTGT)GTACCAACCACTTCCCACTGTTTTCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605183937_hdxg GTGAACCTTATAGGTTGGGCCCTTCCTTCTGG(+CAGTGT)GATCCCTTGATCATATGGGAAGTTCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605183935_pr4l GAGAGCCTAGAGAACTTCCCATATGATCAAGG(+CAGTGT)GATCCCAGAAGGAAGGGCCCAACCTATAAGG NGG 10 8 17 8 24 0 100 5 10
    240605183933_9qv5 AGTCGCAGGAGGCAGGCGTGGCCCTTTCCCAG(+CAGTGT)GATCCTCACAGACAGATGTGGGAAGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605183931_sbnx GATGGCACCTCCTTCCCACATCTGTCTGTGAG(+CAGTGT)GATCCTGGGAAAGGGCCACGCCTGCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605183930_uypv AGGCTTCTATCTGCTCTCAGATGTCTGCTGAG(+CAGTGT)GATCCACCACTCACTGCTCTTGCTCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605183928_1mzu CTCAGCACCCAGAGCAAGAGCAGTGAGTGGTG(+CAGTGT)GATCCTCAGCAGACATCTGAGAGCAGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605183927_44a2 CTGTCACCCAGCACTGGCAAGGAAGACAGGAG(+CAGTGT)GATCCATGAGGCTGACTGGCTAGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605183925_jel8 AGCACTAGGCTTGCTAGCCAGTCAGCCTCATG(+CAGTGT)GATCCTCCTGTCTTCCTTGCCAGTGCTGGGT NGG 10 8 17 8 24 0 100 5 10